Zeinab Anwar Elkabanny
Ain Shams University, Egypt
Title: Screening of Egyptian obese children and adolescents for insertion/deletion polymorphism in angiotensin-converting enzyme gene
Biography
Biography: Zeinab Anwar Elkabanny
Abstract
Screening of Egyptian obese children and adolescents for insertion/deletion polymorphism in angiotensin-converting enzyme gene
Background & Aims: Relatively few studies have examined the contribution of Angiotensin-Converting Enzyme (ACE) candidate genes for development of childhood obesity-hypertension phenotype. Thus, we aimed to screen Egyptian obese children and adolescents for Insertion/Deletion (I/D) polymorphism in the gene encoding ACE and its relation to hypertension.
Methods: One hundred forty two (142) children and adolescents were included (70 with simple obesity and 72 controls). All were subjected to blood pressure measurement, anthropometric assessment and assessment of fasting lipid profile and fasting glucose and insulin. In addition, DNA extraction and genotyping for ACE I/D polymorphism was done.
Results: Obese children had higher frequency of DD genotype (cases 30% versus 11.1% in controls, p=0.01) and D alleles (Cases: 61.8% versus 48.6% in controls, p=0.01) and lower frequency of II genotype (Cases: 27.1% versus 34.7% in controls, p=0.04) and I allele (38.2% versus 51.4% respectively p=0.01) than controls. Also, obese children with hypertension and pre-hypertension had higher frequency of DD genotype and D alleles than II genotype and I alleles. DD genotype and D allele were risk factors for hypertension (OR: 9.86 and 11.57 respectively, p<0.001) while dyslipidemia and insulin resistance were not associated with I/D polymorphism in the ACE gene.
Conclusion: DD genotype and D-allele of I/D polymorphism in the ACE gene were associated with a higher risk of hypertension and pre-hypertension in Egyptian obese children.